Supplementary Data on Substrate Stiffness Effects on Human Gingival Fibroblast Signaling

A 380.4 KB DOCX file from figshare contains supplementary data for a study on how substrate stiffness modulates human gingival fibroblast paracrine signaling. The study, authored by Watcharaphol Tiskratok and last updated in April 2026, investigated the effects on osteogenic differentiation of human periodontal ligament cells under normal and inflammatory conditions. The file is licensed under CC-BY-4.0.

Use Cases

• Analyze cytokine and chemokine expression data from HGFs cultured on substrates of varying stiffness.
• Study the effects of conditioned medium from HGFs on osteogenic marker expression and mineralization in HPDLCs.
• Investigate the role of p38 MAPK and CXCR4 signaling pathways in stiffness-associated cellular responses.
• Examine data on matrix metalloproteinase and tissue inhibitor expression under LPS-induced inflammatory conditions.

Strengths

• Data is associated with a detailed experimental study published on figshare.
• The dataset is openly available under a CC-BY-4.0 license.
• The study includes functional blocking experiments with a CXCR4 inhibitor to establish mechanistic causality.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is a single 380.4 KB DOCX file, indicating a limited scope of supplementary data.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, authored by Watcharaphol Tiskratok.

Collection Method

Data generated from laboratory experiments involving cell culture, qRT-PCR, ELISA, immunofluorescence, and Alizarin red S staining.

Time Range

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Freshness

Last updated 2026-04-15 11:39:19; freshness should be verified.

Geography

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