Synaptosome Calcium Handling and Respiration Data from Cryopreserved MND Motor Cortex

Supplementary file 1_Synaptosomes isolated from cryopreserved MND motor cortex reveal altered calcium handling and reduced complex IV–linked respiration is a dataset by Sean D. Morrison, published on figshare under CC-BY-4.0 license. It likely contains functional measurements from synaptosomes isolated from human and mouse motor cortex, comparing motor neuron disease (MND) samples to controls. The dataset was last updated on May 22, 2026.

Use Cases

• Compare depolarization-evoked calcium influx between MND and control synaptosomes based on the described functional assays.
• Analyze complex IV-driven oxygen consumption in synaptosome-enriched fractions based on Seahorse respiratory assay results.
• Investigate correlations between calcium response amplitudes and donor variables like post-mortem interval based on the described lack of correlation.
• Validate the utility of cryopreserved brain tissue for synaptosome functional studies based on the described methodology.

Strengths

• Dataset includes paired fresh and frozen mouse cortex samples, providing a methodological control.
• Results are derived from both human MND motor cortex samples and a hSOD1G93A mouse model, offering cross-species comparison.
• The dataset is openly available under a CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The data is contained in a DOCX file, which may require extraction or conversion for analysis.

Provenance

Source

Sean D. Morrison

Collection Method

Synaptosomes were isolated from cryopreserved human motor cortex and mouse cortex for functional assays.

Freshness

Last updated 2026-05-22 05:33:42; freshness should be verified.