Synergistic Antibacterial Effect of CATH-2 and D-Amino Acids Against Mastitis Pathogens

A supplementary research document details the synergistic antibacterial effect of combining the antimicrobial peptide CATH-2 with D-amino acids against Gram-positive bacteria causing mastitis in cows. The study includes results from antibacterial assays, growth kinetics, checkerboard synergy tests, and cytotoxicity assessments on mammalian cell lines. The document was authored by Edwin J.A. Veldhuizen and last updated on 2026-05-29.

Use Cases

• Analyzing synergistic interactions between antimicrobial peptides and amino acids based on the described checkerboard assays.
• Evaluating the cytotoxicity of novel antimicrobial combinations on bovine udder epithelial cells based on the described WST, LDH, and trypan blue assays.
• Investigating the efficacy of antimicrobial treatments in raw milk versus laboratory media based on the described results in fresh raw milk.

Strengths

• Includes specific quantitative results such as Fractional Inhibitory Concentration (FIC) indices of 0.37 and <0.12.
• Cytotoxicity testing was performed on multiple mammalian cell lines, including a relevant primary cell type (bovine udder epithelial cells).
• Tested against multidrug-resistant clinical Staphylococcus aureus isolates, indicating relevance to real-world antibiotic resistance.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 1.4 MB file size suggests a small dataset, likely a single document rather than a large data corpus.

Provenance

Source

figshare

Collection Method

Experimental research involving antibacterial and cytotoxicity assays.

Freshness

Last updated 2026-05-29 06:07:29; freshness should be verified.