Supplementary file 1_Systems biology approach unveils the cellular and molecular mechanism

Venkatesh Kumaresan authored a supplementary document analyzing the cellular and molecular mechanisms of formalin-inactivated Coxiella burnetii vaccines in mice. The document, last updated on 2026-06-04, details bulk RNA sequencing and flow cytometry data comparing immune responses between phase I and phase II vaccines. It concludes that sustained neutrophil activation is critical for the protective immunity induced by the phase I vaccine.

Use Cases

• Compare transcriptomic profiles of vaccine-induced immune responses based on bulk RNA sequencing data.
• Analyze the role of neutrophil degranulation and TLR pathways in vaccine efficacy based on gene expression findings.
• Evaluate humoral immune response differences between vaccine types based on phase I-specific IgM and IgG antibody measurements.
• Investigate the impact of neutrophil depletion on vaccine-mediated protection based on challenge experiments.

Strengths

• The document provides a direct comparison of two vaccine types (PIV and PIIV) generated from axenic cultures.
• It integrates multiple analysis methods: bulk RNA sequencing, flow cytometry, humoral response assays, and neutrophil depletion studies.
• The dataset is openly available under a CC-BY-4.0 license.

Limitations

• The dataset is a 1.5 MB DOCX file; the underlying raw data (e.g., sequencing reads, cytometry files) are not directly provided.
• Column-level documentation for any potential embedded data is absent; field semantics must be inferred from the text.
• Row count and data structure are unknown, which limits suitability assessment for direct computational analysis.

Provenance

Source

Venkatesh Kumaresan

Collection Method

Data likely contains results from mouse immunization experiments, RNA sequencing, and flow cytometry performed for the described study.

Freshness

Last updated 2026-06-04 04:27:19