Effects of Filtration and Centrifugation on Gut Microbiota in FMT Preparation

A 2026 study by Yuchi Liu compares fecal microbiota suspension preparation methods. The dataset includes 16S rDNA sequencing results from nine adult volunteer samples processed via filtration and centrifugation at various speeds. It assesses microbial viability, taxonomic composition, and functional potential.

Use Cases

• Comparing microbial viability and mortality based on different centrifugation speeds mentioned in the description
• Analyzing the selective effect of centrifugation on bacterial genera like Delftia and Acinetobacter
• Evaluating impurity removal efficiency of differential centrifugation for FMT preparation
• Benchmarking filtration methods for preserving microbial composition and functional potential

Strengths

• Includes data from nine adult volunteer samples, providing a basis for comparison
• Assesses multiple preparation parameters: filtration alone and centrifugation at various speeds
• Employs multiple analysis methods: live/dead staining, colony forming unit enumeration, and 16S rDNA sequencing

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Sample size of nine volunteers may limit statistical power for some analyses

Provenance

Source

Yuchi Liu via figshare

Collection Method

Fresh fecal samples were processed via filtration and centrifugation; microbial viability was assessed via staining and CFU enumeration; 16S rDNA sequencing was performed.

Freshness

Last updated 2026-05-12 05:43:20