Supplementary Data on S1P and Angiogenesis in Acidic Osteosarcoma Microenvironment

Supplementary file 1 from a study investigating the role of sphingosine-1-phosphate in osteosarcoma-associated angiogenesis under acidic conditions. The 1.1 MB PDF document, authored by Sofia Avnet and last updated on 2026-05-21, contains detailed methods and results from experiments using Matrigel assays, HUVEC cultures, and 3D spheroids. Findings identify an acidosis–S1P axis that promotes endothelial sprouting and tubulogenesis, which was inhibited by the drug FTY720.

Use Cases

• Study the effects of sphingosine-1-phosphate (S1P) on endothelial cell behavior based on the described dose-response experiments.
• Investigate how acidic tumor microenvironments influence pro-angiogenic factor secretion based on the analysis of conditioned media from osteosarcoma spheroids.
• Explore the role of extracellular vesicles in tumor signaling based on the assessment of vesicle-associated mediators like bFGF and Endoglin.
• Evaluate potential therapeutic targets for pathological angiogenesis based on the described inhibition of S1P signaling with FTY720.

Strengths

• The dataset is associated with a detailed, peer-reviewed scientific study describing specific experimental methods and results.
• The file is licensed under CC-BY-4.0, permitting open sharing and adaptation.
• The description provides concrete biological findings, such as S1P's dose-dependent effects on tubulogenesis and sprouting.

Limitations

• The primary data format is a PDF, which may require extraction or manual transcription for computational analysis.
• Column-level documentation and sample data are unavailable, limiting assessment of the underlying data's structure.
• The dataset is small at 1.1 MB, suggesting it is a supplementary document rather than a large primary data repository.

Provenance

Source

figshare

Collection Method

Data likely generated from laboratory experiments including Matrigel-based angiogenesis assays, HUVEC cultures, 3D osteosarcoma spheroids, and microfluidic systems.

Freshness

Last updated 2026-05-21 05:34:02