Supplementary file 1_Understanding multi-azole resistance in Aspergillus fumigatus: the im

Nine azole-resistant Aspergillus fumigatus isolates were identified from 156 soil samples across 13 Indian states. The study investigates resistance mechanisms, including mutations in the cyp51A and hmg1 genes and overexpression of efflux pump genes. The document was authored by Pooja Sen and last updated on 2026-05-28.

Use Cases

• Analyze genetic mutations associated with azole resistance based on sequencing of the cyp51A and hmg1 genes.
• Investigate the role of efflux pump gene overexpression in antifungal resistance based on qRT-PCR data for mdr1, mdr4, mfsC, and cdr1B.
• Study cross-resistance patterns in fungal isolates based on antifungal susceptibility testing against itraconazole, voriconazole, and posaconazole.
• Explore environmental sources of resistant fungi based on the collection of soil samples from multiple geographic regions.

Strengths

• Identifies 9 azole-resistant isolates from a survey of 156 soil samples.
• Reports 18 non-synonymous mutations in the hmg1 gene, including 14 novel ones outside the sterol-sensing domain.
• Analyzes expression levels for multiple efflux pump genes (mdr1, mdr4, mfsC, cdr1B) in resistant isolates.

Limitations

• The dataset is a 25.6 KB DOCX file, suggesting limited scope and likely a supplementary document rather than primary data.
• Row count and column-level documentation are unknown, which may limit suitability assessment.
• Data may reflect geographic bias inherent to the collection from 13 Indian states.

Provenance

Source

figshare

Collection Method

Soil samples were screened, followed by antifungal susceptibility testing, gene sequencing, and qRT-PCR analysis.

Freshness

Last updated 2026-05-28 04:35:56; freshness should be verified.

Geography

13 states across India