Supplementary file 1_Visual–acoustic thigmotaxis in zebrafish larvae: a high throughput NA

Zebrafish larvae at 120 hours post-fertilization were exposed to neuroactive substances and challenged with visual and acoustic stimuli to measure thigmotaxis and locomotor activity. The study compares a 24-well format to a 96-well format, finding equivalent results and supporting higher-throughput screening. This supplementary file, authored by Monica Torres-Ruiz and last updated in May 2026, details the development of a reproducible and sensitive behavioral assay for chemical safety assessment.

Use Cases

• Benchmark dose modeling for neurotoxicity based on thigmotaxis and locomotor endpoints described in the study.
• Comparing assay sensitivity between different well formats (24-round vs. 96-square) for high-throughput behavioral screening.
• Evaluating the effects of specific neuroactive reference compounds (caffeine, diazepam) and additional substances on zebrafish larval behavior.
• Developing a test battery for neurotoxicity assessment by integrating the multiplexed visual–acoustic thigmotaxis assay.

Strengths

• Assay performance was validated with reference compounds, showing caffeine increased and diazepam decreased thigmotaxis under specific stimuli.
• The method is described as reproducible, scalable, and sensitive, with thigmotaxis generally more sensitive than locomotor activity across tested compounds.
• The 24-well and 96-well formats produced equivalent results, supporting the use of the higher-throughput 96-square-well system.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a 432 KB DOCX file, which is a small supplementary document rather than a primary data table.

Provenance

Source

figshare

Collection Method

Experimental assay on zebrafish larvae exposed to compounds and challenged with visual and acoustic stimuli.

Time Range

Experimental time point at 120 hours post-fertilization (hpf).

Freshness

Last updated 2026-05-04 05:30:36; freshness should be verified.

Geography

Laboratory study; geographic origin not specified.