Xtr-Mediated Gene Regulation in Clostridioides difficile

A supplementary research file from figshare, authored by Wenjing Wu and last updated in May 2026. The document details a study on the XRE family transcriptional regulator Xtr in Clostridioides difficile, investigating its role in oxidative and antibiotic stress resistance. It includes results from gene knockout, transcriptome sequencing, and protein binding assays.

Use Cases

• Studying the genetic basis of antibiotic resistance based on the described phenotypic changes in knockout mutants.
• Analyzing transcriptional regulatory networks based on the described binding of Xtr protein to the 4Fe-4S binding protein promoter.
• Investigating bacterial adaptation to oxidative stress based on the described hydrogen peroxide tolerance assays.
• Validating gene function based on the described complementation and reporter gene assays.

Strengths

• The dataset is associated with a detailed research methodology described in the summary, including gene knockout, transcriptomics, and protein binding assays.
• It is published under a permissive CC-BY-4.0 license, allowing for reuse and redistribution.

Limitations

• The dataset is a 35.6 KB DOCX file, which is a very small supplementary document rather than a primary data repository.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for quantitative analysis.

Provenance

Source

figshare

Collection Method

Generated from a laboratory study employing Allele-coupled exchange (ACE) gene knockout, transcriptome sequencing, EMSA, and reporter gene assays.

Freshness

Last updated 2026-05-29 05:53:07; freshness should be verified.