Supplementary file 1_Yiqi Huoxue Yangyin Decoction attenuates diabetic nephropathy in db/db mice by modulating METTL3-mediated m6A methylation of mTOR to restore podocyte autophagy.docx

A 4.2 MB supplementary document details a molecular biology study on Yiqi Huoxue Yangyin Decoction (YHY) for diabetic nephropathy. The research, authored by JinXing He and last updated in April 2026, investigates the formula's chemical metabolites and its mechanism via the METTL3-m6A-mTOR pathway in db/db mice and podocyte cell models.

Use Cases

• Analyze the identified chemical metabolites from UPLC-MS/MS to correlate phytochemical profiles with observed therapeutic effects.
• Study the reported measurements of renal function and podocyte injury markers to model disease progression and treatment response.
• Investigate the quantified relationships between METTL3 expression, m6A methylation levels, mTOR mRNA stability, and autophagic activity for pathway analysis.
• Use the in vivo (db/db mouse) and in vitro (MPC-5 podocyte) experimental results to validate computational models of diabetic nephropathy mechanisms.

Strengths

• Provides a defined chemical metabolite profile of the YHY formula via UPLC-MS/MS analysis.
• Mechanistic investigation spans both an in vivo mouse model and in vitro podocyte cell cultures.
• Data is openly shared under a CC-BY-4.0 license, facilitating reuse.

Limitations

• The 4.2 MB file is a supplementary document, not a structured dataset, limiting direct computational analysis.
• Sample sizes for the mouse and cell models are not specified, making statistical generalizability unclear.
• The data is static and tied to a single study's conclusions, not updated with new findings.

Provenance

Source

figshare, uploaded by study author JinXing He.

Collection Method

Experimental data from UPLC-MS/MS chemical profiling, db/db mouse model studies, and high glucose-stimulated MPC-5 podocyte assays.

Time Range

null

Freshness

null

Geography

Study likely conducted in a laboratory setting; specific geographic location of the research is not provided.