Experimental Autoimmune Myasthenia Gravis Study on Gut Microbiota and Immune Response

A supplementary research document from a study investigating the role of gut microbiota in experimental autoimmune myasthenia gravis (EAMG) in Lewis rats. The file, authored by Wenqi Tian and last updated in April 2026, describes methods including 16S rRNA sequencing, ELISA cytokine measurement, and flow cytometry. Results detail changes in microbial composition, serum biomarkers, and intestinal barrier proteins.

Use Cases

• Analyze the relationship between gut microbiota dysbiosis and autoimmune disease progression based on 16S rRNA sequencing data mentioned in the description
• Investigate Th17/Treg cell imbalance in myasthenia gravis models based on flow cytometry results described
• Study intestinal barrier integrity markers like Claudin-1 and Muc2 in autoimmune conditions based on immunofluorescence analysis
• Correlate serum inflammatory cytokines (IFN-γ, TNF-α, IL-17, IL-10) and AChR-Ab levels with disease severity based on ELISA measurements

Strengths

• Study design includes multiple experimental models (EAMG, antibiotic-induced dysbiosis, fecal microbiota transplantation) for comparative analysis
• Employs several laboratory techniques (16S rRNA sequencing, ELISA, immunofluorescence, flow cytometry) for multi-faceted data generation
• Released under a permissive CC-BY-4.0 license for reuse

Limitations

• The dataset is a 10.5 KB DOCX file, indicating it is a supplementary document rather than a primary data repository
• Row count and column-level documentation are absent; field semantics must be inferred from the text
• Data may reflect bias inherent to the specific experimental model (Lewis rats) used

Provenance

Source

figshare

Collection Method

Data generated from laboratory experiments on Lewis rats, including immunization, sequencing, and assay techniques as described.

Time Range

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Freshness

Last updated 2026-04-10 05:29:21; freshness should be verified

Geography

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