Supplementary file 2_Faecal inflammatory protein markers in children with autism spectrum

57 children with autism spectrum disorder (ASD) and 57 of their biological siblings without ASD were compared for fecal levels of α1-antitrypsin, immunoglobulin A, and calprotectin. The data was generated by Joško Osredkar and last updated on 2026-04 15. Samples were analyzed using UHPLC–MS/MS after careful participant screening to control for confounding factors.

Use Cases

• Compare gut inflammatory protein levels between ASD and control groups based on sibling-matched design.
• Analyze correlations between protein markers and ASD severity based on Childhood Autism Rating Scale (CARS) scores.
• Explore subgroup patterns in moderate versus severe ASD based on IgA levels and S100A8/S100A9 ratios.
• Validate or challenge meta-analysis findings on gut inflammation in ASD based on quantified fecal protein data.

Strengths

• Sibling-matched design controls for shared environmental and microbiome factors.
• Participants were screened to exclude recent antibiotic use, GI infections, and abnormal diets.
• Protein quantification used internal standards and normalization to total protein for accuracy.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Subgroup analyses were underpowered (e.g., severe subgroup: n = 11 pairs) and exploratory.

Provenance

Source

Joško Osredkar via figshare.

Collection Method

Stool samples were processed via freeze-drying, protein extraction, and analyzed by UHPLC–MS/MS.

Time Range

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Freshness

Last updated 2026-04-15 09:17:44; freshness should be verified.

Geography

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