Supplementary file 2_Integrated transcriptomics identifies ER stress–associated apoptosis

Dl-3-n-butylphthalide (NBP) administration within 120 minutes post-resuscitation was associated with reduced kidney injury markers in a porcine traumatic cardiac arrest model. The dataset integrates bulk transcriptomic, microarray, and single-cell RNA sequencing data to investigate endoplasmic reticulum stress-associated apoptosis pathways in acute kidney injury. Kechun Zhou published this supplementary file under a CC-BY-4.0 license on figshare in June 2026.

Use Cases

• Validate endoplasmic reticulum stress and unfolded protein response gene signatures based on integrated bulk and single-cell transcriptomics.
• Assess Dl-3-n-butylphthalide's impact on kidney function based on serum creatinine and blood urea nitrogen measurements.
• Investigate proximal tubule cell state progression and apoptosis based on pseudotime inference and PT-state stratification.
• Correlate molecular readouts like PERK, CHOP, and caspase-3 expression with histopathological injury scores.

Strengths

• Integrated analysis of bulk transcriptomic, microarray, and single-cell RNA sequencing data provides a multi-layered view of kidney injury.
• In vivo validation in a porcine model includes physiological, histological, and molecular endpoints measured at multiple time points up to 24 hours.
• The dataset is associated with a specific, time-sensitive therapeutic intervention (NBP administered within 120 minutes of ROSC).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 24-hour observation window provides only early-stage data on injury and intervention effects.

Provenance

Source

figshare

Collection Method

Data generated from integrated transcriptomic analysis and an in vivo porcine model of traumatic cardiac arrest.

Freshness

Last updated 2026-06-04 05:33:32; freshness should be verified.