CircRNA Landscape of Intrauterine Adhesions: ceRNA Axes and Splicing Usage

1,724 high-confidence circRNAs from 1,143 host genes were identified in a reanalysis of RNA sequencing data from intrauterine adhesions and controls. The dataset, created by Yingqing Chen and published on figshare in May 2026, integrates ceRNA circuits and circRNA-associated splicing usage linked to uterine contractility and immunity.

Use Cases

• Construct competing endogenous RNA (ceRNA) networks based on identified circRNA-miRNA-mRNA circuits.
• Analyze circRNA-associated splicing usage shifts in pathways related to uterine contractility and epithelial-mesenchymal transition.
• Correlate circRNA expression with macrophage phenotype signatures derived from immune deconvolution.
• Validate dysregulated circRNAs and their putative target mRNAs using back-splice junction-spanning RT-qPCR.
• Explore RNA-binding protein co-expression networks linked to circRNA-associated splicing events.

Strengths

• Dataset integrates results from four independent circRNA callers for a high-confidence catalog.
• Includes validation results from an independent clinical cohort using RT-qPCR and Western blotting.
• Links circRNA expression to specific functional modules like smooth muscle contractility and macrophage phenotypes.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is 657 KB, indicating a limited scope focused on analysis results rather than raw sequencing data.

Provenance

Source

Reanalysis of rRNA-depleted RNA sequencing data from IUA and controls (GSE224093).

Collection Method

CircRNA assembly using four independent callers, differential expression analysis, ceRNA circuit construction, and splicing usage quantification.

Freshness

Last updated 2026-05-07 04:37:45; freshness should be verified.