Supplementary File 2: Mucinase Discovery for Pseudomyxoma Peritonei Therapy

A research document details the discovery of mucinase enzymes from Bacteroides thetaiotaomicron for degrading pathological mucus in pseudomyxoma peritonei (PMP). The file is 2.4 MB, authored by Jun Liu, and was last updated on May 1, 2026. It describes an integrated workflow from probiotic protease discovery to functional validation of mucinases BT4244, BT3015, and BT4272.

Use Cases

• Identify potential mucolytic enzyme targets based on the described transcriptomic analysis of Bacteroides thetaiotaomicron.
• Design minimally invasive therapeutic strategies for mucinous cancers based on the described microbial protease workflow.
• Study the role of PULs gene clusters and M60 family metalloproteinases in mucus degradation as outlined in the document.

Strengths

• The document provides a detailed, integrated workflow from discovery to functional validation of specific enzymes.
• It is licensed under CC-BY-4.0, allowing for broad reuse and adaptation.
• The research focuses on a specific, clinically challenging condition (pseudomyxoma peritonei).

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Likely contains research findings and analysis from cultivating Bacteroides thetaiotaomicron with patient-derived mucus.

Freshness

Last updated 2026-05-01 05:24:45; freshness should be verified.