CRISPR Tadpole Brain Activity and Transcriptome Data for DEE48 Epilepsy Study

7.6 MB of data from a Xenopus laevis CRISPR model investigates AP3B2 gene loss in Developmental and Epileptic Encephalopathy 48. The dataset includes measurements of locomotor activity, seizure-like episodes, neural Ca2+ activity, interhemispheric synchrony, whole-brain transcriptomes, and blood-brain barrier integrity. It was generated by Sulagna Banerjee and published on figshare in March 2026 under a CC BY 4.0 license.

Use Cases

• Analyze interhemispheric synchrony patterns in forebrain and midbrain Ca2+ activity data from ap3b2 CRISPants versus controls.
• Identify downregulated gene pathways from whole-brain transcriptome comparisons, focusing on ion transport, axon guidance, and GABA neurotransmission.
• Correlate locomotor hyperactivity and seizure-like episode frequency with blood-brain barrier leakage assay results using sodium fluorescein.
• Assess the phenotypic rescue effect by comparing neural activity and locomotor data from losartan-treated and untreated CRISPant cohorts.

Strengths

• Data originates from a controlled CRISPR/Cas9 gene-editing experiment targeting the AP3B2 ortholog in Xenopus laevis.
• Includes multi-modal measurements: behavioral (locomotor activity), physiological (GCaMP6s Ca2+ imaging), transcriptomic (RNA-seq), and functional (BBB assay).
• Published under a permissive CC BY 4.0 license, facilitating open reuse and redistribution.

Limitations

• Dataset size is 7.6 MB, indicating a relatively small-scale experimental study, not a large-scale population dataset.
• Specific data structure, column names, and row counts are unknown, complicating immediate analytical use.
• Data is specific to a Xenopus tadpole model organism, limiting direct translational insights to human DEE48 without further validation.

Provenance

Source

figshare, authored by Sulanna Banerjee.

Collection Method

Generated via CRISPR/Cas9 targeting of the ap3b2.S gene in Xenopus laevis tadpoles, with subsequent behavioral, imaging, transcriptomic, and assay-based analysis.

Freshness

Last updated March 2026, indicating recent data.