Xtr-Mediated Regulation of 4Fe-4S Binding Protein in Clostridioides difficile

A supplementary research document from figshare, authored by Wenjing Wu and last updated on 2026-05-29. It details experimental findings on the role of the Xtr transcriptional regulator in Clostridioides difficile, including its impact on hydrogen peroxide tolerance and antibiotic susceptibility. The 2.8 MB file contains results from gene knockout, transcriptome sequencing, EMSA, and reporter gene assays.

Use Cases

• Study transcriptional regulation of stress response genes based on the described Xtr knockout and complementation mutants.
• Investigate links between iron-sulfur cluster proteins and antibiotic resistance based on the 4Fe-4S binding protein gene findings.
• Analyze bacterial tolerance mechanisms to oxidative stress based on the hydrogen peroxide tolerance assays.
• Model virulence regulatory networks in pathogenic bacteria based on the described changes in toxin gene expression and cellular toxicity.

Strengths

• Specific experimental results are cited, such as a 3.69-fold downregulation of the 4Fe-4S_BP gene.
• The methodology is detailed, including the use of Allele-coupled exchange (ACE) gene knockout, transcriptome sequencing, EMSA, and reporter gene assays.
• The dataset is shared under a permissive CC-BY-4.0 license.

Limitations

• The dataset is a 2.8 MB DOCX file, which is a small document format not directly machine-readable as structured data.
• Column-level documentation and sample data are unavailable, requiring manual inspection of the document to extract structured information.
• The row count and specific data tables are unknown, limiting suitability assessment for computational analysis.

Provenance

Source

figshare

Collection Method

Experimental research involving gene knockout, phenotypic analysis, transcriptomics, EMSA, and reporter gene assays.

Freshness

Last updated 2026-05-29 05:53:08.