Complement-Mediated ADCP Cytotoxicity Analysis in Primary Mouse Macrophages

A 3.6 MB PDF file authored by Carly M. Van Wagoner and last updated on 2026-04 13. The document presents a quantitative analysis of antibody-dependent cellular phagocytosis (ADCP) via Fcγ and complement pathways in primary mouse macrophages, using live-cell imaging and genetic disruption. It establishes complement-mediated ADCP as a distinct, finite, and additive cytotoxic mechanism for monoclonal antibodies.

Use Cases

• Modeling phagocytic pathway kinetics based on the described temporal dynamics of Fcγ and complement ADCP.
• Investigating macrophage effector exhaustion based on the described dose-dependent and reversible states of hypophagia.
• Designing antibody therapies based on the described additive and non-redundant effects of engaging both ADCP pathways.

Strengths

• Data is openly licensed under CC-BY-4.0.
• Analysis is based on primary mouse macrophages and quantitative live-cell imaging, as described in the methods.
• Findings provide a functional framework comparing two distinct phagocytic pathways (Fcγ and complement).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a single 3.6 MB PDF, which may contain processed results rather than raw experimental data.

Provenance

Source

Carly M. Van Wagoner via figshare.

Collection Method

Quantitative live-cell imaging of primary mouse macrophages, genetic disruption of Fcγ receptor signaling, and controlled modulation of complement activity.

Time Range

null

Freshness

Last updated 2026-04-13 05:48:25; freshness should be verified.

Geography

null