Supplementary file 3_Integrated transcriptomics identifies ER stress–associated apoptosis

A supplementary file from a 2026 study investigating Dl-3-n-butylphthalide's effects on acute kidney injury following traumatic cardiac arrest in pigs. The document integrates bulk transcriptomic and single-cell RNA sequencing data with in vivo validation results from a porcine model. It was authored by Kechun Zhou and shared under a CC-BY-4.0 license.

Use Cases

• Validate transcriptomic signatures of endoplasmic reticulum stress-associated apoptosis based on integrated bulk and single-cell RNA-seq data.
• Benchmark drug efficacy metrics for Dl-3-n-butylphthalide based on serum creatinine and blood urea nitrogen measurements.
• Analyze proximal tubule cell state progression during ischemia-reperfusion injury based on pseudotime inference results.
• Correlate protein expression levels (PERK, CHOP, caspase-3) with histological injury scores from H&E and TUNEL staining.

Strengths

• Integrates multiple data modalities including bulk transcriptomics, single-cell RNA sequencing, and in vivo porcine model validation.
• Provides specific experimental details such as NBP dosage (2.5 mg/kg) and measurement timepoints (1, 2, 4, and 24 hours).
• Focuses on a specific cell type (proximal tubule cells) through PT-state stratification and pseudo-bulk analysis.

Limitations

• The dataset is a 17.7 MB DOCX file, which is a small and unstructured format for quantitative analysis.
• Row count and column-level documentation are unknown, limiting suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Generated from integrated transcriptomic analyses and in vivo experiments in a porcine traumatic cardiac arrest model.

Freshness

Last updated 2026-06-04 05:33:30