Transcriptomic and Behavioral Data from CRISPR Tadpole Model of DEE48

Experimental data from a Xenopus laevis CRISPR model investigates the loss of the AP3B2 gene in Developmental and Epileptic Encephalopathy 48. The dataset includes whole-brain transcriptomes, locomotor activity measurements, and neural calcium imaging from ap3b2.S knockout tadpoles and sibling controls. It supports analysis of altered gene pathways, seizure-like behavior, and blood-brain barrier integrity.

Use Cases

• Compare whole-brain transcriptomes between ap3b2 CRISPants and controls to identify downregulated pathways like ion transport and GABA neurotransmission.
• Analyze locomotor activity data to quantify increased seizure-like episodes in ap3b2.S knockout tadpoles.
• Use GCaMP6s calcium imaging data to assess increased interhemispheric synchrony in forebrain and midbrain neural activity.
• Investigate blood-brain barrier integrity by correlating transcriptomic pathway data with sodium fluorescein leakage assay results.
• Evaluate the effects of losartan treatment on locomotor hyperactivity and neural activity in the CRISPR model.

Strengths

• Data originates from a controlled CRISPR/Cas9 gene targeting experiment in Xenopus laevis, a model organism for neurodevelopmental studies.
• Includes multi-modal data: behavioral (locomotor activity), imaging (GCaMP6s calcium signals), and molecular (whole-brain transcriptomes).
• Released under a permissive CC BY 4.0 license, facilitating open reuse and analysis.

Limitations

• The dataset is small at 8.0 MB, suggesting limited raw data volume for large-scale machine learning training.
• Specific row counts, column structures, and sample data are unavailable, complicating initial assessment of data shape and completeness.
• Data is derived from a non-mammalian tadpole model, which may limit direct translational relevance to human DEE48.

Provenance

Source

Figshare, authored by Sulagna Banerjee.

Collection Method

Generated via CRISPR/Cas9 targeting of the ap3b2.S gene in Xenopus laevis tadpoles, with data collected on locomotor activity, calcium imaging, transcriptomics, and a blood-brain barrier assay.

Freshness

Last updated in March 2026.