NT5E Gene Expression Analysis for Pulmonary Hypertension Biomarker Discovery

Supplementary data from a 2026 bioinformatics study identifies NT5E as a potential diagnostic marker for pulmonary hypertension. The analysis includes gene expression data from the GSE113439 and GSE53408 datasets sourced from the Gene Expression Omnibus. Author Yanan Chu published the supporting results on figshare.

Use Cases

• Analyze NT5E gene expression levels and their correlation with pulmonary hypertension progression.
• Evaluate diagnostic accuracy using ROC curve data, including AUC values of 0.891 and 0.894 from specific GEO datasets.
• Investigate immune cell infiltration associations, such as between NT5E expression and plasma cells or eosinophils.
• Perform gene set enrichment analysis (GSEA) on pathways linked to high NT5E expression in PH.
• Validate bioinformatics findings with in vitro experimental data on pulmonary arterial smooth muscle cell behavior.

Strengths

• Includes validated ROC analysis with AUC values reaching 0.894 for NT5E.
• Integrates data from multiple established sources: GEO, KEGG, and Reactome databases.
• Supplementary material for a peer-reviewed study methodology, including WGCNA and CIBERSORT analyses.

Limitations

• Small dataset at 1.8 MB, indicating limited raw data volume.
• Unknown row count and specific column structure for the provided XLS file.
• Findings are specific to pulmonary hypertension and two identified NMRGs (NT5E, CD38), limiting generalizability.

Provenance

Source

Gene Expression Omnibus (GEO) datasets GSE113439 and GSE53408, with NMRGs from KEGG and Reactome.

Collection Method

Bioinformatics analysis using Limma R package, weighted gene co-expression network analysis (WGCNA), and CIBERSORT algorithm, validated by in vitro cellular experiments.

Freshness

Last updated on 2026-04-06.