CRISPR Tadpole Model Data for DEE48 Epilepsy Research

3.0 MB of supplementary data from a Xenopus laevis CRISPR model investigating AP3B2 gene loss. The dataset contains results on seizure-like activity, interhemispheric synchrony, brain transcriptomics, and blood-brain barrier integrity from the study. It was authored by Suljana Banerjee and last updated in March 2026.

Use Cases

• Analyze patterns of increased interhemispheric synchrony in neural activity data from CRISPR-edited tadpoles.
• Investigate transcriptomic downregulation in pathways related to GABA neurotransmission and ion transport.
• Model the relationship between blood-brain barrier leakage and seizure susceptibility using sodium fluorescein assay results.
• Correlate locomotor hyperactivity and spontaneous neural activity measurements from GCaMP6s sensor data.

Strengths

• Data is derived from a controlled CRISPR/Cas9 gene editing experiment targeting the AP3B2 ortholog.
• Includes multi-modal measurements: behavioral (locomotor activity), neural (GCaMP6s imaging), transcriptomic, and physiological (BBB assay).
• Published under a permissive CC BY 4.0 license for open reuse.

Limitations

• The dataset is a 3.0 MB supplementary document (DOCX), not a structured, machine-readable table, limiting direct computational analysis.
• Sample size (number of tadpoles or biological replicates) is not specified in the provided metadata.
• Data is specific to the Xenopus laevis model organism, which may limit direct translational insights for human DEE48.

Provenance

Source

figshare

Collection Method

Generated via CRISPR/Cas9 gene editing of Xenopus laevis (ap3b2.S CRISPants) and comparative analysis with unedited sibling controls.

Freshness

Last updated on 2026-03-23.