Complement-Mediated ADCP Cytotoxicity Analysis in Primary Mouse Macrophages

776.5 KB supplementary PDF from a 2026 figshare study by Carly M. Van Wagoner. The file details a quantitative live-cell imaging analysis comparing two phagocytic pathways—Fcγ receptor-dependent and complement-dependent ADCP—in primary mouse macrophages. Findings establish complement-mediated ADCP as a distinct, high-capacity cytotoxic mechanism with additive effects and distinct exhaustion kinetics.

Use Cases

• Modeling macrophage cytotoxic capacity and exhaustion based on the described kinetics of Fcγ and complement pathways.
• Designing antibody therapies to leverage additive effects based on the finding of functional non-redundancy between pathways.
• Investigating phagocytic pathway bypass mechanisms based on the observation that cADCP can proceed after fADCP exhaustion.

Strengths

• Published under a permissive CC-BY-4.0 license.
• Describes a quantitative live-cell imaging methodology with genetic and activity controls.
• Focuses on a distinct cytotoxic mechanism (cADCP) with established temporal dynamics and capacity.

Limitations

• Data is presented as a 776.5 KB PDF; underlying numerical or image datasets are not directly accessible.
• Row count and column-level documentation are unknown.
• The dataset's scope is limited to the specific experimental findings described.

Provenance

Source

figshare, author Carly M. Van Wagoner.

Collection Method

Quantitative live-cell imaging of primary mouse macrophages with genetic and complement activity modulation.

Time Range

Experimental timeframe not specified.

Freshness

Last updated 2026-04-13.

Geography

Laboratory study; geographic scope not specified.