NT5E Gene Expression Analysis for Pulmonary Hypertension Diagnosis

94.0 KB Excel file contains bioinformatics results identifying NT5E as a key NAD+ metabolism-related gene in pulmonary hypertension. Author Yanan Chu published this supplementary data in April 2026, supporting a research article that validated findings through in vitro cellular experiments. The file includes results from ROC curve analysis, gene set enrichment, and immune infiltration assessments across multiple GEO datasets.

Use Cases

• Validate NT5E expression as a diagnostic biomarker using provided ROC curve AUC values of 0.891 and 0.894.
• Analyze the association between NT5E expression levels and immune cell infiltration, specifically plasma cells and eosinophils.
• Correlate high NT5E expression with enriched biological pathways from the Gene Set Enrichment Analysis (GSEA) results.
• Use the identified NMRG list, including NT5E and CD38, for cross-referencing in other pulmonary hypertension transcriptomic studies.

Strengths

• Diagnostic accuracy for NT5E is quantified with specific AUC values from two independent datasets (GSE113439 and GSE53408).
• Analysis integrates multiple data sources: GEO database, KEGG, Reactome, and weighted gene co-expression network analysis.
• Findings are supported by in vitro experimental validation of NT5E's functional role in pulmonary arterial smooth muscle cells.

Limitations

• The 94.0 KB file size indicates a very limited dataset, likely containing only summary results rather than raw expression data.
• Unknown row and column counts prevent assessment of the underlying data's volume and granularity.
• Analysis is confined to specific GEO datasets (GSE113439, GSE53408), which may limit generalizability.

Provenance

Source

Gene Expression Omnibus (GEO) database, Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome databases.

Collection Method

Bioinformatics analysis using Limma R package for differential expression, weighted gene co-expression network analysis, and CIBERSORT algorithm for immune infiltration.

Freshness

Last updated April 2026.