Supplementary Data for CRISPR-Cas9 DNA Repair Study in the Two-Spotted Spider Mite

Sander De Rouck provides supplementary files for a study on DNA repair pathways in the chelicerate Tetranychus urticae. The 145.6 MB ZIP file, last updated in May 2026, contains data supporting the functional analysis of double-strand break repair and the enhancement of homology-directed repair via Polθ disruption. The dataset is licensed under CC-BY-4.0 and hosted on figshare.

Use Cases

• Analyzing the efficiency of homology-directed repair based on the reporter assay targeting the phytoene desaturase gene.
• Comparing DNA repair outcomes between Polθ-deficient and wild-type strains based on the described functional analysis.
• Investigating the fitness cost of disrupting polθ for genome engineering applications based on the described modest fitness impact.

Strengths

• Dataset size is 145.6 MB, indicating a medium-scale supplementary data archive.
• The description provides specific biological findings, such as the redirection of repair toward homology-directed repair in Polθ-deficient strains.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely contains experimental data from CRISPR-Cas9 and reporter assays in Tetranychus urticae.

Freshness

Last updated 2026-05-28 16:01:35; freshness should be verified