Sestrin2 in C2C12 Myotubes: Supplementary Images for Cellular Stress Study

14.2 MB of supplementary image files for a 2026 study on sestrin proteins in skeletal muscle cells. The data, authored by Jiani Qian and shared under a CC-BY-4.0 license, includes PNG files supporting research on cellular stress responses in C2C12 myotubes. The study investigates the role of sestrin2 under endoplasmic reticulum stress and its impact on mTORC1 signaling pathways.

Use Cases

• Validate protein expression levels of sestrins 1-3 based on Western blot images.
• Analyze cellular stress markers like Ddit3 and ATF4 from induced C2C12 myotubes.
• Study the effect of sestrin2 siRNA silencing on mTORC1 effector S6K1 phosphorylation.
• Compare myotube morphology and size under different stress conditions (e.g., tunicamycin, H2O2).

Strengths

• Data is directly linked to a peer-reviewed scientific article, providing context.
• Files are shared under a permissive CC-BY-4.0 license for reuse.
• Last updated on 2026-04-17, indicating recent availability.

Limitations

• Row count and column-level documentation are absent; data structure must be inferred from images.
• The 14.2 MB size suggests a small-scale, supplementary dataset.
• Data is limited to in vitro C2C12 myotube experiments; in vivo applicability is unknown.

Provenance

Source

figshare, authored by Jiani Qian.

Collection Method

Experimental data generated from C2C12 myotube cell culture studies.

Freshness

Last updated 2026-04-17 11:51:27