Endothelial Cell Proteome Changes After Bag3 Deletion Across Tissues

This dataset contains label-free LC-MS/MS proteomics data from endothelial cells (ECs) isolated from brain, heart, lung, and peripheral skeletal muscle (SkM) tissues of adult mice (22+ weeks old) with endothelial-specific Bag3 deletion (KO) and wild-type controls (WT). Data reveals tissue-specific baseline EC proteomes and altered mitochondrial complex abundances in heart ECs. KO most significantly affects SkM EC proteome, increasing 31 proteins (e.g., Mybpc2) and decreasing 65 proteins (e.g., Cdc42).

Use Cases

• Compare endothelial cell proteomes across four mouse tissues.
• Assess tissue-specific effects of Bag3 deletion on the EC proteome.
• Identify mitochondrial proteome differences between tissue-specific ECs.
• Analyze proteins upregulated/downregulated in SkM ECs after Bag3 KO.

Strengths

• Proteomics data from FACS-purified ECs (CD31+/CD146+) from multiple tissues.
• Direct comparison of WT EC proteomes reveals 367-570 differentially abundant proteins between tissue pairs.
• Mitochondrial proteome analysis shows heart ECs dedicate higher proportions to complexes I, III, IV, and V.
• Bag3 KO shows strongest effect on SkM EC proteome with 96 significantly altered proteins (31 up, 65 down).

Limitations

• Sample size per group/tissue not specified in description.
• Column-level metadata (e.g., protein IDs, abundance values) requires download for full assessment.
• Freshness unknown beyond 2026-05-09 upload date.
• Scale limited by unknown row count.

Provenance

Source

figshare (10.6084/m9.figshare.27947639)

Collection Method

Endothelial cells isolated by FACS (CD31+/CD146+) from tissues of adult Cdh5(PAC)-CreERT2;Bag3f/f mice treated with tamoxifen (KO) or vehicle (WT). LC-MS/MS label-free proteomics performed on equal numbers of male and female mice.

Time Range

Data collected from mice >22 weeks old.

Geography

Mouse model data; no explicit geography.