Human Pluripotent Stem Cell-Derived Macrophages in Kidney Organoids: A Preliminary Study

Supplementary material for a study investigating the effects of adding human pluripotent stem cell-derived macrophages to kidney organoids. The study, authored by figshare admin karger, assesses macrophage integration and its impact on nephrogenesis markers like synaptopodin and nephrin. The dataset was last updated on May 22, 2026.

Use Cases

• Analyze the correlation between macrophage quantity and organoid development based on the described immunostaining quantification.
• Study the spatial distribution of macrophages relative to endothelia based on CD31/PECAM-1 and CD68 co-immunostaining results.
• Investigate the dose-dependent effects of macrophages on glomerular marker expression based on changes in synaptopodin and nephrin immunostaining areas.
• Assess technical feasibility for populating nephron organoids with maturing macrophages as described in the methodology.

Strengths

• Dataset is licensed under CC-BY-4.0, permitting broad reuse with attribution.
• The description provides specific methodological details, including macrophage addition ratios of 1%, 5%, and 20%.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The study is described as preliminary, and findings require replication with other hPSC lines.

Provenance

Source

figshare

Collection Method

Experimental study combining hPSC-derived macrophages with hPSC-derived kidney precursors.

Freshness

Last updated 2026-05-22 09:55:12; freshness should be verified.