IFNLR1 Variant Signaling Data from HEK293T and iHep Cells

4.1 MB of supplementary data from a study comparing signal transduction by full-length and truncated interferon lambda receptor-1 (IFNLR1) variants. The dataset, published under CC-BY-4.0 on figshare, includes results from proximity ligation assays, flow cytometry, western blotting, and gene expression profiling in HEK293T and stem cell-derived hepatocyte models. It was last updated on 2026-06-01.

Use Cases

• Compare JAK1 and TYK2 phosphorylation levels based on IFNLR1 variant expression data.
• Analyze differential interferon-stimulated gene (ISG) induction patterns based on signaling mediator utilization.
• Model the impact of receptor internalization rates on downstream gene expression based on experimental results.
• Investigate the role of noncanonical IFNLR1 variants in TYK2-signaling dependence for antiviral responses.

Strengths

• Data is associated with a detailed methodological description including Duolink assay, ImageStream flow cytometry, and western blotting.
• Includes comparative analysis of two distinct IFNLR1 splice variants (variant 1 and variant 2).
• Results are derived from multiple cell models: HEK293T cells, wild-type, and IFNLR1 knock-out stem cell-derived hepatocytes.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The 4.1 MB size suggests a small dataset, potentially limiting statistical power for some analyses.

Provenance

Source

figshare

Collection Method

Experimental data from proximity ligation assays, flow cytometry, western blotting, inhibitor susceptibility tests, and gene expression profiling.

Freshness

Last updated 2026-06-01 07:15:02; freshness should be verified.