Supplementary Material for Rapid hiPSC-to-Neuron Conversion Protocol

A 1.0 MB PDF contains supplementary material for a protocol converting human induced pluripotent stem cells (hiPSCs) into functional neurons in as few as 6 days. The protocol uses a tetracycline-inducible Neurogenin-2 system and minimal media, developed by an author on figshare and last updated in April 2026. The material likely includes methodological details, results from immunocytochemistry, RT-qPCR, and multielectrode array recordings showing network activity by day 35.

Use Cases

• Replicating a low-cost, growth-factor-free neuronal induction protocol based on the described TET-on NGN2 system.
• Analyzing neurite outgrowth dynamics based on the 24-hour live imaging of Td-Tomato labelled cells mentioned in the description.
• Studying functional neuronal maturation based on multielectrode array recordings of spontaneous and synchronous network activity.
• Comparing neuronal conversion efficiency based on the use of the Notch inhibitor DAPT as described in the methods.

Strengths

• Protocol achieves neuronal conversion in as few as 6 days, a rapid timeframe.
• Multielectrode array recordings demonstrated synchronous network firing by day 35, indicating functional maturation.
• Released under a permissive CC-BY-4.0 license, allowing for broad reuse.

Limitations

• The dataset is a 1.0 MB PDF; the underlying raw experimental data (e.g., image files, quantitative recordings) are not provided.
• Row count and column-level documentation are unknown, which may limit suitability assessment for computational analysis.
• Description metadata is limited; actual data quality and completeness require manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely contains supplementary experimental data and detailed methodology from the associated research protocol.

Freshness

Last updated 2026-04-30 05:55:15; freshness should be verified.