Supplementary Material: Neutrophil APC Phenotype After Streptococcus Infection

The dataset contains flow cytometry data from a 2026 study investigating the immunomodulatory role of neutrophils infected with Streptococcus pyogenes (GAS). Human neutrophils were infected with wild-type GAS or mutants lacking streptolysins and co-cultured with autologous CD4⁺ T cells. Results show infected neutrophils acquire antigen-presenting cell-like characteristics and partially activate T cells, revealing a novel mechanism in streptococcal infections.

Use Cases

• Analyzing neutrophil activation markers (CD15, CD66b, HLA-DR, CD40, CD86) post-bacterial infection.
• Comparing intracellular bacterial killing efficiency between different GAS mutant strains.
• Evaluating CD4⁺ T cell activation (CD25, cytokine release) in response to infected neutrophils.
• Investigating the dose-dependent effect of GAS infection on neutrophil phenotype.

Strengths

• Dataset is openly licensed under CC-BY-4.0, facilitating reuse.
• Cross-platform presence on figshare indicates established dissemination.
• The experimental design is detailed, including specific bacterial strains (5448, Δslo, ΔsagA) and cell types.

Limitations

• Column names and row count are not provided, limiting precise structural understanding.
• The dataset consists solely of supplementary material in PDF format, which may not be directly analyzable as raw data.
• The single file size (1716286) is given, but the data's internal structure is unknown.

Provenance

Source

figshare admin karger

Collection Method

Human neutrophils were infected with GAS and analyzed via flow cytometry after infection alone or after co-culture with autologous CD4⁺ T cells.

Freshness

Last updated on 2026-03-24.