RT-PCR Primer Sequences for Xenopus Embryo Gene Expression Study

Primer sequences for quantitative real-time PCR analysis of gene expression in Xenopus laevis embryos. The data supports a study on stage-specific GABA-dependent regulation during axis elongation. It was authored by Masaki Hachiman and uploaded to figshare in April 2026.

Use Cases

• Designing RT-PCR assays by referencing specific primer sequences for target genes.
• Validating gene expression normalization strategies using the provided reference genes odc1 and gapdh.
• Replicating the experimental setup for measuring expression during gastrula and tailbud stages.

Strengths

• Primer sequences are directly linked to a specific, peer-reviewed study on Xenopus embryo development.
• Data is provided in a structured XLSX format (362.9 KB), facilitating direct use in experimental design.

Limitations

• The dataset is very small (362.9 KB), containing only primer sequences without accompanying gene expression measurements.
• Application is highly specialized, limited to researchers working with Xenopus laevis embryos during specific developmental stages.

Provenance

Source

figshare, authored by Masaki Hachiman.

Collection Method

Primer sequences used for quantitative real-time PCR (qRT-PCR) in the associated study.

Time Range

Covers the gastrula and tailbud developmental stages of Xenopus laevis embryos.

Freshness

Last updated on 2026-04-05.