Differentially Expressed Genes in Xenopus Embryo Posterior Explants

Stage 26 Xenopus laevis embryo posterior dorsal explants provide data on transcriptional changes after GABA signaling inhibition. The dataset lists differentially expressed genes identified via RNA-seq from two biological replicates, each pooling tissue from three embryos. Author Masaki Hachiman published this data in 2026 to support the study of GABA-dependent gene regulation during axial elongation.

Use Cases

• Identify candidate genes regulated by GABA signaling by analyzing the list of differentially expressed genes (DEGs).
• Validate RNA-seq findings from the associated study by examining the provided DEG log fold changes and p-values.
• Compare transcriptional responses between control and picrotoxin-treated conditions using the gene expression metrics.
• Investigate gene function during tailbud-stage axial elongation by filtering DEGs based on expression magnitude and significance.

Strengths

• Data is derived from a controlled experimental design with two biological replicates per condition.
• Each biological replicate pools explants from three embryos, reducing individual embryo variability.
• Dataset is explicitly linked to a peer-reviewed study on stage-specific GABA regulation.

Limitations

• The dataset is small at 395.6 KB, indicating a limited gene list rather than full expression matrices.
• Only two biological replicates limit statistical power for detecting subtle expression changes.
• Analysis is restricted to a single developmental stage (stage 26) and tissue type (posterior dorsal explant).

Provenance

Source

Figshare, authored by Masaki Hachiman.

Collection Method

RNA-seq analysis performed on posterior dorsal explants from Xenopus laevis embryos treated with 0.5 mM picrotoxin for 3 hours.

Time Range

Embryos at developmental stage 26.

Freshness

Last updated in April 2026.