Supporting Data for Aptamer-Mediated MMP14 Targeting in Osteoporosis Therapeutics

Supporting data for a PhD project aiming to establish a novel aptamer-mediated DNA nanotechnology platform for targeted gene silencing in osteoporosis treatment. The data likely contains results from Systematic Evolution of Ligands by Exponential Enrichment (SELEX) experiments and evaluations of DNA nanostructure delivery efficiency. The dataset was authored by Yinuo Xie and last updated on 2026-04-23.

Use Cases

• Analyze aptamer binding affinity and specificity based on SELEX selection rounds and candidate characterization mentioned in the description
• Evaluate DNA nanostructure internalization efficiency based on flow cytometry results for tetrahedron, icosahedron, and tesseract structures
• Identify potential protein targets for aptamers based on proximity labeling and mass spectrometry analysis described
• Investigate endocytosis pathways for nanocarrier uptake based on the described clathrin-mediated endocytosis and macropinocytosis

Strengths

• Includes specific quantitative results, such as the binding affinity (33.19 nM) of the Apt15 aptamer candidate
• Describes a structured experimental approach with three main objectives: aptamer selection, nanocarrier optimization, and functional evaluation
• Results are validated using multiple techniques, including SELEX, flow cytometry, and mass spectrometry

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Description metadata is limited; actual data quality requires manual inspection after download

Provenance

Source

figshare

Collection Method

Experimental data from Systematic Evolution of Ligands by Exponential Enrichment (SELEX), flow cytometry, and mass spectrometry analysis.

Time Range

Experimental timeframe not specified.

Freshness

Last updated 2026-04-23 07:51:28; freshness should be verified

Geography

Geographic coverage not specified.