DNA-Encoded Library Screening Data for YEATS2 Inhibitor Discovery

Shihao Liu published supporting data for the selection of DNA-encoded libraries against epigenetic protein targets on figshare in May 2026. The dataset contains results from high-throughput screening of a library with over 500 million unique members against the YEATS2 YEATS domain. It includes validated hits, with binding affinities confirmed via SPR and MST assays, and the most potent hit exhibiting a dissociation constant (Kd) in the range of 0.47–7.3 μM.

Use Cases

• Identify novel small-molecule inhibitors based on DNA-encoded library enrichment data.
• Analyze competitive binding patterns based on screening groups designed around a known peptide inhibitor.
• Validate binding affinities and structure-activity relationships based on SPR and MST assay results.
• Compare on-DNA and off-DNA synthesis validation approaches for hit compounds.

Strengths

• Screening library contains over 500 million unique members, indicating high chemical diversity.
• Binding affinities for validated hits are confirmed with dissociation constants (Kd) all below 20 μM.
• Data includes results from both competitive and novel binding site screening strategies.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

High-throughput screening using DNA-encoded library technology, followed by synthesis and validation via SPR and MST assays.

Time Range

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Freshness

Last updated 2026-05-06 09:23:59; freshness should be verified.

Geography

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