Human Patellar Tendon Proteomics Over Three Symptom Months

Human patellar tendon biopsies from participants with early-stage unilateral patellar tendinopathy were analyzed via data-independent acquisition liquid chromatography–mass spectrometry. The dataset contains proteomic profiles from symptomatic and contralateral tendons across one, two, and three months of symptom duration. Protein- and peptide-level abundances were quantified and assessed using two-way ANOVA.

Use Cases

• Identify protein-level changes over time by analyzing the 12 proteins and 14 peptides with significant main effects from symptom duration.
• Compare proteomic profiles between symptomatic and nonsymptomatic contralateral tendons using quantified peptide and protein abundances.
• Perform temporal proteomic profiling to investigate early molecular changes within the first three months of tendinopathy.
• Apply statistical models like two-way ANOVA to assess factors of symptom status and symptom duration on protein abundance.

Strengths

• Data captures a specific early disease window, profiling tendons within the first three months of symptom duration.
• Proteomic analysis includes both peptide- and protein-level quantified abundances from a bottom-up workflow.
• Findings are derived from human patellar tendon biopsies, providing direct clinical relevance.

Limitations

• The dataset is small, with a file size of 625.0 KB, indicating limited scope and sample size.
• No detectable proteomic difference was found between symptomatic and contralateral tendons, limiting comparative analysis power.
• Sample details like participant count, row count, and specific column structure are not provided.

Provenance

Source

Danielle Steffen via figshare.

Collection Method

Biopsies collected from participants with unilateral patellar tendinopathy and analyzed by data-independent acquisition liquid chromatography–mass spectrometry.

Time Range

Symptom duration of one, two, and three months.

Freshness

Last updated on 2026-03-23.