Proteomic Profiles of Human Patellar Tendons in Early Tendinopathy

A proteomics dataset contains protein and peptide abundance measurements from human patellar tendon biopsies. The data was collected by Danielle Steffen to analyze changes across one, two, and three months of symptom duration in participants with early-stage unilateral patellar tendinopathy. The analysis identified 12 proteins and 14 peptides with significant time-dependent changes.

Use Cases

• Perform differential abundance analysis on the 12 significant proteins to model their change over symptom duration.
• Investigate the correlation between the 14 significant peptides and their corresponding parent proteins for biomarker validation.
• Apply statistical models like two-way ANOVA to test for interaction effects between symptom status and symptom duration using the quantified protein abundances.
• Compare proteomic profiles between symptomatic and contralateral tendons to study systemic effects in early tendinopathy.

Strengths

• Data is derived from a controlled human biopsy study focusing on the first three months of a specific pathology.
• Analysis identified a specific set of 12 proteins and 14 peptides with statistically significant changes over time.
• Dataset includes paired samples from symptomatic and contralateral nonsymptomatic tendons for within-subject comparison.

Limitations

• Sample size is not provided, which limits assessment of statistical power for the reported findings.
• The 4.2 MB file size suggests a relatively small dataset, potentially containing aggregated results rather than raw spectral data.
• Temporal coverage is limited to a maximum of three months post-symptom onset, capturing only early-stage disease.

Provenance

Source

figshare, authored by Danielle Steffen.

Collection Method

Data-independent acquisition liquid chromatography–mass spectrometry (DIA LC-MS) on tendon biopsies from participants with unilateral patellar tendinopathy.

Time Range

Symptom duration of one, two, and three months.

Freshness

Last updated March 2026.