Synthesis and Application of a Suite of 2,5-Aryl Tetrazole Photoaffinity-Based Probes for

Jack Russo's dataset on figshare contains chemoproteomic profiles from applying a suite of 2,5-aryl tetrazole photoaffinity probes to defined bacterial cultures and human fecal lysates. The data, last updated in June 2026, demonstrates enrichment of glycoside hydrolases and other carbohydrate-associated proteins. The 1.5 MB XLSX file provides a substrate-centric view of glycan-processing protein networks.

Use Cases

• Identify carbohydrate-associated protein engagement based on probe enrichment profiles described in the study.
• Compare glycan-processing module activity between defined bacterial cultures and complex fecal lysates as mentioned in the description.
• Complement abundance-based proteomics by analyzing protein organization around glycan substrates using the tetrazole probe suite.
• Profile microbial carbohydrate and mucin metabolism networks in gut microbiota using the described photoaffinity-based chemoproteomics strategy.

Strengths

• Data is derived from both defined bacterial cultures and complex human fecal lysates, providing comparative context.
• The dataset is focused on a specific methodological approach using 2,5-tetrazoles as photoreactive groups.
• File size is 1.5 MB, indicating a manageable, focused dataset.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale analyses.
• The dataset is licensed under CC-BY-NC-4.0, restricting commercial use.

Provenance

Source

figshare

Collection Method

Photoaffinity-based chemoproteomics using synthesized monosaccharide-bearing probes.

Freshness

Last updated 2026-06-03 03:03:55; freshness should be verified.