Evaluation of Plasma Protein Enrichment Methods with Orbitrap Astral DIA

Proteonano confidently identified 299 proteins with mapped blood concentrations below 10^6 pg/L, while immunodepletion yielded 660 confident proteins in the moderate abundance range. This dataset compares the performance of seven depletion and enrichment technologies for platelet-free plasma proteomics, authored by Salem Al Siblani and last updated in March 2026. The evaluation assessed proteomic depth, protein quantification precision, and functional analysis using an Orbitrap Astral instrument with data-independent acquisition.

Use Cases

• Benchmarking protein enrichment methods based on the comparison of seven technologies described.
• Analyzing the trade-off between proteome coverage and repeatability based on the reported protein counts and coefficients of variation.
• Investigating the impact of sample preparation (platelet-free vs. platelet-rich plasma) on results based on the described deviation from prior studies.

Strengths

• Includes specific performance metrics, such as Proteonano identifying 299 low-abundance proteins and immunodepletion covering 660 proteins.
• Reports quantitative precision with repeatability metrics, including a median coefficient of variation of 13% for MagNet-SAX.
• Compares multiple methods (7 technologies) on consistent metrics: proteomic depth, quantification precision, and functional analysis.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 10.4 KB, indicating limited scope, likely containing summary results rather than raw experimental data.

Provenance

Source

figshare

Collection Method

Experimental evaluation using an Orbitrap Astral instrument with data-independent acquisition (DIA) on platelet-free plasma.

Time Range

Experimental date range is not specified.

Freshness

Last updated 2026-03-17 14:05:25; freshness should be verified.

Geography

Geographic coverage is not specified.