Paramecium Bursaria Growth and Microbiome Data in Defined vs. Lettuce Media

A 2023 clonal strain and a publicly maintained strain of Paramecium bursaria were cultured in a defined synthetic algal medium (AF-6) and conventional lettuce infusion. The dataset likely contains measurements of cell proliferation, size, compression-induced extension, symbiotic algal abundance, and 16S rRNA gene sequencing results for host-associated bacterial communities. It was authored by Yuri Matsushima and published on figshare under a CC-BY-4.0 license.

Use Cases

• Compare host growth and physiology based on culture medium type mentioned in the description
• Analyze symbiotic algal community composition based on rbcL metataxonomic analysis results
• Investigate bacterial microbiome stability based on 16S rRNA gene sequencing data across different media
• Benchmark experimental reproducibility in microbiome research based on the defined medium framework

Strengths

• Includes data from two distinct Paramecium bursaria strains (an unfed clonal strain established in 2023 and a publicly maintained algae-fed strain)
• Compares multiple physiological metrics: cell proliferation, size, compression-induced extension, and symbiotic algal abundance
• Uses two molecular analysis methods (rbcL metataxonomics and 16S rRNA gene sequencing) to characterize microbial communities

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is small (27.8 KB), indicating a limited scope of observations

Provenance

Source

figshare

Collection Method

Experimental laboratory culturing and molecular sequencing, as described.

Freshness

Last updated 2026-05-01 05:24:40; freshness should be verified