Murine Sepsis Model Chromatin States in Myeloid-Derived Suppressor Cells

A high-resolution epigenetic map profiles DNA methylation and chromatin accessibility at gene promoters in splenic myeloid-derived suppressor cells from a murine sepsis model. The data includes young and older adult male and female mice, with unsupervised clustering identifying nine distinct promoter classes reflecting chromatin dynamics. Transcriptomic profiling links these promoter accessibility states with gene expression.

Use Cases

• Analyze age- and sex-dependent sepsis-induced promoter opening (Classes 1-4) to identify regulatory mechanisms of immune dysfunction.
• Investigate persistent promoter closure with varying DNA methylation levels (Classes 5-7) for epigenetic silencing patterns post-sepsis.
• Correlate promoter accessibility states from MAPit-FENGC data with single-cell RNA sequencing results to validate expression links.
• Compare chromatin dynamics in splenic CD11b+Gr1+ cells between young and older adult cohorts to study aging effects on sepsis response.

Strengths

• Data generated using MAPit-FENGC, a single-molecule assay providing simultaneous DNA methylation and chromatin accessibility profiles.
• Analysis includes nine distinct promoter classes, providing a detailed categorization of chromatin dynamics.
• Study design incorporates both young and older adult male and female mice, enabling age and sex comparisons.
• Findings are corroborated by independent transcriptomic profiling, linking epigenetic states with gene expression.

Limitations

• The dataset is small (53.6 KB), indicating limited scope and likely a summary or processed results rather than raw sequencing data.
• Data is derived from a specific murine model, limiting direct translation to human sepsis pathophysiology.
• The input does not specify row or column counts, sample sizes, or raw data availability, hindering assessment of statistical power.

Provenance

Source

Angel M. Charles via figshare.

Collection Method

MDSCs were isolated from spleens in a murine sepsis model and analyzed using MAPit-FENGC and single-cell RNA sequencing.

Freshness

Last updated March 24, 2026.