Plasmodiophora Brassicae Isolate Data with Genetic Purity and Virulence Profiles

A 29.4 KB Excel file from figshare, last updated April 13, 2026, authored by Ling Cao. The dataset presents results from a novel method for producing high-purity single protoplast-derived isolates of the clubroot pathogen. It includes infection success rates, genetic homogeneity assessments, and avirulence gene frequencies for evaluating pathogen strains.

Use Cases

• Assessing infection success rates of different Plasmodiophora brassicae strains based on the described SPI method
• Evaluating genetic purity of pathogen isolates based on reported SNP homogeneity and whole-genome sequencing results
• Identifying pathogen races and avirulence gene frequencies for resistance gene deployment strategies
• Comparing the efficiency of the single-protoplast-derived isolate method against traditional single spore-derived isolates

Strengths

• Infection success rates for the SPI method are reported, ranging from 77.8% to 100%
• Genetic homogeneity of isolates is quantified, with SPIs showing ≤ 1.0% genomic heterogeneity
• Avirulence gene frequencies for six resistance genes (Rcr1 to Rcr10) are provided for 18 SPIs

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data may reflect methodological bias inherent to the specific experimental protocol described

Provenance

Source

figshare, authored by Ling Cao

Collection Method

Data likely generated from laboratory experiments involving enzymatic digestion of infected root tissue, isolate purification, and genetic analysis.

Time Range

Experimental timeframe not specified in the description.

Freshness

Last updated 2026-04-13 05:48:28; freshness should be verified

Geography

Geographic origin of the pathogen strains (e.g., AB11, AB16) is not specified.