MPCE Assay Results for Hypervirulent Klebsiella Pneumoniae Genotyping

A multiplex fluorescence PCR-capillary electrophoresis (MPCE) system for detecting 16 genetic markers in hypervirulent Klebsiella pneumoniae. The assay, validated against next-generation sequencing, achieved a limit of detection of 10² copies/μL and processed results in 152 minutes. The dataset, authored by Huimin Ning and last updated in April 2026, is shared under a CC-BY-4.0 license.

Use Cases

• Rapid identification of hypervirulent Klebsiella pneumoniae strains based on the detection of five core virulence genes.
• Simultaneous profiling of antimicrobial resistance genes to inform treatment decisions.
• High-throughput genotyping for epidemiological investigations based on capsular serotype and resistance markers.
• Validation of novel molecular diagnostic assays against next-generation sequencing results.

Strengths

• Assay validated against next-generation sequencing with strong concordance (κ = 0.659–1.000).
• Simultaneously detects 16 genetic markers with a reported limit of detection of 10² copies/μL.
• Demonstrated no cross-reactivity against a panel of non-target pathogens.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (18.7 KB), indicating limited scope, likely containing assay protocol or summary results rather than extensive raw data.

Provenance

Source

figshare

Collection Method

Multiplex fluorescence PCR-capillary electrophoresis (MPCE) assay developed in a research study.

Time Range

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Freshness

Last updated 2026-04-20 05:23:03; freshness should be verified.

Geography

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