Rickettsia Rickettsii Detection Data from RPA-CRISPR/Cas12a Assay

An isothermal RPA–CRISPR/Cas12a assay targeting the vut gene can detect 60–70 copies of Rickettsia rickettsii DNA in about 40 minutes. The dataset, authored by Sezayi Ozubek and shared on figshare under CC-BY-4.0, includes results from specificity testing on four R. rickettsii strains and archived canine whole-blood DNA extracts from six experimentally infected dogs.

Use Cases

• Benchmarking rapid molecular detection limits based on the reported 60–70 copy sensitivity.
• Training models to differentiate R. rickettsii strains based on specificity testing data.
• Analyzing temporal detection patterns in canine hosts using longitudinal blood extract results.
• Validating isothermal amplification workflows for pathogen detection in clinical samples.

Strengths

• Assay demonstrated no cross-reactivity with a panel of non-target spotted fever group rickettsiae and tick-borne bacteria.
• Detection limit is quantified at 60–70 copies per reaction using synthetic DNA standards.
• Results include data from archived longitudinal canine whole-blood DNA extracts (n=6).

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Row count is unknown, which may limit suitability assessment.
• Performance in canine blood was intermittent and limited to specific post-infection days, as noted in the description.

Provenance

Source

figshare, author Sezayi Ozubek.

Collection Method

Experimental assay results from an established RPA–CRISPR/Cas12a workflow.

Time Range

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Freshness

Last updated 2026-04-16 05:18:55; freshness should be verified.

Geography

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