Bovine PRNP Gene Indel Polymorphism Genotyping Results for 62 Cattle Products

62 commercial cattle-derived products were genotyped for two insertion/deletion polymorphisms in the bovine PRNP gene promoter and intron 1. The dataset, authored by Ye Xu and last updated in 2026, contains results from a duplex MIRA-LFD assay, a visual detection method developed to be simpler and faster than DNA sequencing. The genotyping results for both loci were reported to be completely consistent with sequencing results.

Use Cases

• Genotyping cattle for BSE susceptibility based on PRNP gene indel polymorphisms mentioned in the description
• Validating rapid visual detection assays against conventional sequencing results
• Performing haplotype analysis for bovine PRNP gene based on simultaneous detection of two polymorphic loci

Strengths

• Genotyping results for both loci were reported to be completely consistent with sequencing results for all 62 samples
• The described assay is noted for being simple, convenient, highly specific, and easy to operate

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The dataset is small in scale at 676.9 KB

Provenance

Source

Ye Xu via figshare

Collection Method

Multienzyme isothermal rapid amplification (MIRA) combined with lateral flow dipstick (LFD) technology

Freshness

Last updated 2026-05-01 05:28:03