Angelica Sinensis Extract Bioactivity for Skin Cell Models

A 15.5 KB document details experimental results from treating human keratinocytes and dermal fibroblasts with Angelica sinensis extract. The study assessed antioxidant, anti-senescent, and antimicrobial effects against skin-relevant microbes like Staphylococcus aureus and Cutibacterium acnes. Authored by Isabella Giacomini and published on figshare in 2026, it provides quantitative readouts for NF-κB nuclear localization, reactive oxygen species levels, and collagen type I alpha 1 immunofluorescence.

Use Cases

• Analyze the concentration-dependent inhibition of Cutibacterium acnes and Malassezia globosa from 24-hour broth microdilution tests.
• Model the relationship between extract concentration (10, 30, 50 μg/mL) and reduction of UVB-induced senescence-associated β-galactosidase in keratinocytes.
• Correlate reactive oxygen species detection levels at 3 and 24 hours with the extract's antioxidant activity under hydrogen peroxide challenge.
• Evaluate the increase in collagen type I alpha 1 (COL1A1) immunofluorescence signal in dermal fibroblasts as a matrix-supportive metric.

Strengths

• Provides specific quantitative results for multiple bioactivity assays, including reactive oxygen species detection at 3 and 24 hours.
• Tests the extract against four specific skin-relevant microbes: Staphylococcus aureus, Staphylococcus epidermidis, Cutibacterium acnes, and Malassezia globosa.
• Uses two established human cell models (HaCaT keratinocytes and BJ-5ta fibroblasts) for in vitro validation.
• Released under the permissive CC BY 4.0 license, facilitating reuse and distribution.

Limitations

• The dataset is very small (15.5 KB), containing only summary results in a DOCX file without underlying raw data or tabular structure.
• No columnar data or sample data is available, limiting direct computational analysis and statistical modeling.
• The experimental scope is limited to specific in vitro cell models and does not include clinical or advanced skin model data.

Provenance

Source

figshare, authored by Isabella Giacomini.

Collection Method

Laboratory experiments on human cell lines, including assays for NF-κB translocation, reactive oxygen species, senescence, collagen immunofluorescence, scratch-wound migration, and broth microdilution.

Time Range

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Freshness

Last updated in March 2026, indicating recent publication.

Geography

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