Ageratina Adenophora Extract Effects on Ascaris Suum Egg Transcriptome and Infectivity

A 2026 study by Luyang Wang investigates the impact of Ageratina adenophora aqueous extract on Ascaris suum egg development. The dataset includes embryonation rates from six extract concentrations (0.10% to 5.00%) and transcriptome analysis identifying 281 to 1,083 differentially expressed genes. It provides a theoretical basis for developing plant-based agents targeting the egg stage of this parasitic nematode.

Use Cases

• Analyze dose-dependent inhibition of nematode embryonation based on six extract concentration levels.
• Investigate disrupted metabolic pathways in parasite eggs based on GO and KEGG enrichment analysis results.
• Validate RNA-Seq findings via qRT-PCR based on key downregulated genes like CYP44A1 and GST-4.
• Assess correlation between transcriptomic changes and reduced infectivity based on larval counts and lesion severity in mice.

Strengths

• Includes specific embryonation inhibition rates, with the strongest effect (34.24%) at the 5.00% concentration.
• Transcriptome analysis quantifies 281 and 1,083 differentially expressed genes for two treatment comparisons.
• Biological findings are validated by in vivo infectivity tests in mice and qRT-PCR.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (17.9 KB), indicating limited scope.

Provenance

Source

Luyang Wang via figshare

Collection Method

In vitro treatment of Ascaris suum eggs with plant extract followed by RNA sequencing and in vivo mouse infection assays.

Freshness

Last updated 2026-04-22 12:34:43; freshness should be verified.