Bovine Muscle Cell Response to GABA and Temperature Stress

A 1.0 MB dataset from figshare documents the effects of γ-Aminobutyric acid (GABA) on bovine skeletal muscle satellite cells. The data, authored by Abid Manzoor and last updated in April 2026, includes results from proliferation assays, gene expression analysis, and transcriptomic profiling under heat and cold stress. It is shared under a CC-BY-4.0 license.

Use Cases

• Modeling the dose-response relationship of GABA on cell proliferation based on CCK-8 and EdU assay results.
• Analyzing gene expression changes in stress-related pathways like Nrf2/Keap1/HO-1 under different temperature conditions.
• Investigating transcriptomic pathway enrichment (e.g., p53, PI3K-Akt, FoxO) in response to thermal stress.
• Correlating in vitro molecular targets with in vivo animal growth metrics from the rumen-protected GABA trial.

Strengths

• Includes data from multiple experimental methods: RT-qPCR, Western blot, immunofluorescence, and transcriptomic analysis.
• Specific dose concentrations (0.5 and 1 mM GABA) and temperature conditions (41°C and 4°C) are defined for mechanistic experiments.
• Results are linked to an in vivo trial using rumen-protected GABA under different Temperature Humidity Index conditions.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The primary data is contained within a DOCX file, which may require extraction and formatting for direct analysis.

Provenance

Source

figshare, author Abid Manzoor.

Collection Method

Experimental data from laboratory studies on bovine skeletal muscle satellite cells.

Freshness

Last updated 2026-04-14 04:14:20; freshness should be verified.