Early Transcriptional Response of Mouse Macrophages to Salmonella Dublin Infection

1,080 differentially expressed mRNAs and 23 miRNAs were identified in RAW 264.7 mouse macrophages 2 hours after infection with Salmonella Dublin. Zi Wang published this high-throughput sequencing dataset on figshare in May 2026 under a CC-BY-4.0 license. The data highlights significant enrichment in TNF, HIF-1, and MAPK signaling pathways.

Use Cases

• Identify key immune regulators based on differentially expressed mRNAs like Acod1, Ccl22, and Bcl2a1b.
• Analyze miRNA-mediated gene regulation during early infection based on up-regulated miR-146-3p and miR-150-5p.
• Investigate signaling pathway activation based on KEGG enrichment results for TNF, HIF-1, and MAPK pathways.
• Validate sequencing results using RT-qPCR data mentioned in the description.
• Study the early transcriptional mechanism of macrophage response to a host-adapted Salmonella pathogen.

Strengths

• Identifies 1,080 differentially expressed mRNAs (492 up, 588 down) and 23 differentially expressed miRNAs (18 up, 5 down).
• Results were verified using RT-qPCR, suggesting validation of the sequencing data.
• Focuses on a specific, early time point (2 hours post-infection) for mechanistic insight.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small (17.9 KB), suggesting limited scope or that the file may contain summary tables rather than raw sequencing data.

Provenance

Source

figshare

Collection Method

High-throughput sequencing of mRNA and miRNA expression in infected vs. uninfected RAW 264.7 mouse macrophages.

Time Range

Data captures the transcriptional state 2 hours after infection.

Freshness

Last updated 2026-05-04 05:33:08; freshness should be verified.