Antarctic Microbial Protein Extracts for Cell and Viral Cryopreservation

22 Antarctic bacterial and yeast isolates were characterized and screened for freezing resistance. Intracellular and extracellular protein-rich extracts from four selected strains were evaluated for cryoprotective activity in E. coli, HEK293 cells, and adenovirus systems over 30–60 days. The dataset, authored by Joana Camila Lopes and last updated in April 2026, highlights Antarctic microorganisms as a source of natural cryoprotective compounds.

Use Cases

• Screening novel cryoprotectants based on Antarctic microbial protein extracts mentioned in the description
• Evaluating cell viability post-freezing based on the described survival rates for E. coli and HEK293 cells
• Assessing viral titer stability during cold storage based on the adenovirus preservation results
• Comparing cryoprotective efficacy of intracellular versus extracellular protein extracts from different microbial strains

Strengths

• Includes data from 22 characterized Antarctic microbial isolates
• Reports specific survival rate increases, such as a 3% viability boost in E. coli with a specific extract
• Evaluates cryoprotective activity across three biological systems: prokaryotic, eukaryotic, and viral

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The dataset is small (675.1 KB), indicating limited scope

Provenance

Source

figshare

Collection Method

Laboratory study involving microbial isolation, protein extraction, and viability assays.

Freshness

Last updated 2026-04-28 15:14:46; freshness should be verified

Geography

Antarctic microorganisms